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	Provedor de dados BABT (2) Autor Llamoca-Zárate,Romulo Marino (2) Bloch Jr.,Carlos (1) Campos,Francisco A. P. (1) Campos,Francisco de Assis Paiva (1) Costa,Itayguara Ribeiro da (1) Landsmann,Joerg (1) Ponte,Luiz Ferreira Aguiar (1) Souza,Petrônio Augusto Simão de (1) Mais... Palavra-chave Cactaceae (2) 2S albumins (1) Cereus jamacaru (1) Mandacarú (1) Opuntia ficus-indica (1) Particle bombardment (1) Prickly-pear (1) Seed proteins (1) Transient gene expression (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2001 (1) 1999 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Biolistic-mediated transient gene expression in shoot apical meristems of the prickly-pear (Opuntia ficus-indica) BABT Llamoca-Zárate,Romulo Marino; Ponte,Luiz Ferreira Aguiar; Landsmann,Joerg; Campos,Francisco de Assis Paiva. We have demonstrated the transient expression of the GUS gene in cells of the meristematic apical dome of Opuntia ficus-indica. DNA delivery into the cells was achieved using a biolistic PDS-1000He instrument from Bio-Rad Laboratories. The transforming DNA was coated in tungsten particles with diameter of 1.3 m m and the distance between the flying disk and the target tissue was 7.5cm and the shooting pressure was adjusted to 1200 psi. This is the first demonstration that the biolistic transformation system can be used to express a transgene in a member of the Cactaceae. Tipo: Info:eu-repo/semantics/article Palavras-chave: Opuntia ficus-indica; Prickly-pear; Cactaceae; Transient gene expression; Particle bombardment. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300005 Isolation and Characterisation of a Reserve Protein from the Seeds of Cereus jamacaru (Cactaceae) BABT Costa,Itayguara Ribeiro da; Souza,Petrônio Augusto Simão de; Bloch Jr.,Carlos; Llamoca-Zárate,Romulo Marino; Campos,Francisco A. P.. We describe here the isolation and characterisation of a major reserve protein from the seeds of Cereus jamacaru. (Cactaceae). This protein has a molecular mass of 5319 kDa and was isolated by a combination of gel filtration chromatography and reverse phase HPLC. The amino acid composition of the protein was determined and it was shown to have similarities with the amino acid composition of several proteins from the 2S albumin storage protein family. The usefulness of this protein as a molecular marker in the Cactaceae is also discussed. Tipo: Info:eu-repo/semantics/article Palavras-chave: Cereus jamacaru; Mandacarú; Cactaceae; Seed proteins; 2S albumins. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000400002 Registros recuperados: 2 Primeira ... 1 ... Última

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